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. 2024 Aug 1;19(8):1189–1204. doi: 10.1016/j.stemcr.2024.07.003

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© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Flt3-expressing ancestry of LT-RUs in the iST-HSC population

(A) Overview of experimental design.

(B–E) (B) (i) Gating strategy used to investigate YFP expression in CD45-expressing iLT-HSCs and iST-HSCs in E14.5 Flt3-cre:flox-YFP FLs. (ii) Quantification of YFP expression (% recombination). n = 10 embryos, 3 litter, 3 experimental days. Exact p values shown. Reconstitution after 4 (C) and 16 (D) weeks. Sufficient numbers of iLT-HSC YFP+ cells could not be purified for transplantation. (i) Contribution to peripheral blood monocytes (Mono), B cells (B), and T cells (T). (ii) Reconstitution of peripheral blood platelet (Plts). Only comparisons where p < 0.05 are displayed. iLT-HSC CD45⁺ YFP−, n = 6 recipients, donor cells from pooled FL from 3 litters, 3 experimental days. iST-HSC CD45⁺ YFP−, n = 7 recipients, donor cells from pooled FL from 5 litters, 5 experimental days. iST-HSC CD45⁺ YFP+, n = 4 recipients, donor cells from pooled FL from 4 litters, 4 experimental days.

(E) Model of co-contribution of eMPPs (Patel et al., 2022) and iST-HSC[LT-RU]s to ongoing adult hematopoiesis. For C and D, Donor-derived reconstitution (Reconstitution %) determined using CD45.2 expression and YFP expression when possible.