Figure 1.

rhTNF-α and rhIL-1β do not influence the generation of hRGCs

Schematic representation of hRGCs generation from human retinal progenitor cells (hRPCs^POU4F2+tdT) using the stage-specific differentiation protocol involving chemically defined medium (CDM) in the presence of rhTNF-α and rhIL-1β (A). Western blot analysis revealed expression of immunoreactive bands corresponding to TNFα-1R1 and IL-1βR1 in hRGCs. The levels of their expression increased in the presence of rhTNF-α and rhIL-1β (B). There was no significant difference in the number of tdT⁺ and PAX6⁺ hRGCs (C and D) and expression levels of hRGCs regulatory gene POU4F2 (E) in different culture conditions, demonstrating that the presence of rhTNF-α and rhIL-1β does not affect hRGC generation. Experiments were carried out in triplicates per group from 3 to 4 independent biological replicates. Values expressed as mean ± SEM. Scale bar: 50 μm.