Figure 2.

rhTNF-α and rhIL-1β influence hRGC neuritogenesis

Analysis of TUJ1⁺ neurites of hRGCs generated in three different conditions revealed a significant increase in the complexities (A and B) and length (A and C) of the processes in the presence of rhTNF-α and rhIL-1β, versus control and their abrogation in antibody-neutralized conditions. Similarly, analysis of SMI32⁺ and TAU1⁺ hRGCs axons revealed a significant increase in their complexities (D and E) and length (D and F) in the presence of rhTNF-α and rhIL-1β, versus control and their abrogation in antibody-neutralized conditions. Examination of the expression of transcripts corresponding to GAP43, SOX11, and KLF6 revealed their levels increased in the presence of rhTNF-α and rhIL-1β, versus control and its abrogation in antibody-neutralized conditions (G). Experiments were carried out in triplicates per group from 3 independent biological replicates. Values expressed as mean ± SEM. Scale bar: 20 μm.