Figure 3.

hμG are generated from human iPSCs by stage-specific recapitulation of developmental mechanism

Schematic representation of stage-specific hμG differentiation protocol with bright-field representation of cells in different stages of differentiation (A). Mature cells expressed immunoreactivities corresponding to μG-specific markers CX3CR1, P2RY12, and TREM2 along with IBA1 (B), and the number of cells expressing these markers was in the excess of 80% of total cells (C). Mature cells elaborated TNF-α and IL-1β when challenged with LPS (D) and migrated in response to μG chemoattractant ADP, which is abrogated in the presence of P2RY12 receptor antagonist, PSB0739 (E). Mature cells phagocytosed fluorescently tagged Aβ_1-42 peptide (F). Experiments were carried out in triplicates per group from 3 independent biological replicates. Values expressed as mean ± SEM. Scale bar: 25 (AIV, B, F) 50 (AIII, E), and 100 μm (AI, AII).