Figure 3.

Loss of AhR in Rorc-expressing cells leads to decrease IL-22 production by ILC3s, not Th22 cells. Flow cytometry was performed on colonocytes isolated from the lamina propria from the following groups: LM Vehicle (n=4), LM I3C (n=4), LM DSS+Vehicle (n=4), LM DSS+I3C (n=4), AhR^ΔRorc DSS+Vehicle (n=4), and AhR^ΔRorc DSS+I3C (n=4). Groups consisted of equal numbers of female and male samples. (A) Representative flow plots for ILC3s, defined as CD45⁺Lin^-CD90.2⁺RORgT(Rorγt)⁺IL-22⁺. (B) Dot plot depicting percentage of IL-22 producing ILC3s. (C) Dot plot depicting total cell number of IL-22 producing ILC3s. (D) Representative flow plots for Th22 cells, defined as CD45⁺Lin⁺CD4⁺IL-22⁺. (E) Dot plot depicting percentage of Th22 cells. (F) Dot plot depicting total cell number of Th22 cells. Error bars equal the standard error mean (SEM). For dot plots, significance was determined using one-way ANOVA and Tukey’s multiple comparisons test. (***p<0.005).