Figure 4.

Loss of AhR in Rorc-expressing cells prevents regulation of Bacteroides during colitis after I3C treatment in female mice. 16S rRNA was performed on colonocytes isolated from the lamina propria of female mice from the following experimental groups: LM Vehicle (n=3), LM I3C (n=2), LM DSS+Vehicle (n=3), LM DSS+I3C (n=3), AhR^ΔRorc DSS+Vehicle (n=4), and AhR^ΔRorc DSS+I3C (n=4). One sample in the AhR^ΔRorc DSS+Vehicle group was excluded since it fell below the threshold of 10,000 reads. (A) Shannon index for alpha diversity. (B) Principal Component Analysis (PCoA) for beta diversity. (C) Heatmap depicting percent OTUs of most significantly altered bacterial genera (left-side); Heatmap depicting significantly altered bacteria genera when comparing two different groups (right-side; significance determined with unpaired, two-tailed t test; box highlighted in red denotes significance as p<0.05). Dot plots are shown depicting percent OTUs for (D) Bacteroides and (E) Peptococcaceae. Error bars equal the standard error mean (SEM). For dot/violin plots, significance was determined using one-way ANOVA and Tukey’s multiple comparisons test unless otherwise indicated. (*p<0.05, **p<0.01, ***p<0.005).