Skip to main content
. 2024 Aug 20;18:1424719. doi: 10.3389/fnins.2024.1424719

Figure 2.

Figure 2

CART treatment protects NSCs and enhances proliferation of NSCs in oxygen and glucose deprivation. (A) CART55-102 (0.8 nM) treatment inhibits loss of neural stem cell structures (Nestin, Red) and increases Nestin (Red) expression in cultured NSCs. Scale bars = 100 μm. (B) CART55-102 (0.8 nM) treatment enhances Sox-2 (Green) expression in cultured NSCs. Scale bars = 50 μm. (C) Quantitative analysis of Nestin fluorescence results using ImageJ software, date was presented as mean ± SEM, ***p < 0.001, ###p < 0.001. (D) Quantitative analysis of. Sox-2 fluorescence results using ImageJ software, date was presented as mean ± SEM, ***p < 0.001, ##p < 0.01. (E) EdU staining detects proliferation of oxygen and glucose deprived NSCs. Scale bars =100 μm. (F) Quantitative analysis of fluorescence results using ImageJ software, date was presented as mean ± SEM, ***p < 0.001, ###p < 0.001. Nestin: Neuroepithelial stem cell protein; Sox-2: Sex determining region Y box 2.