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. 2024 Aug 20;15:1406804. doi: 10.3389/fimmu.2024.1406804

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Copyright © 2024 Siegel, Padamsey, Bolender, Hargreaves, Fraidling, Ducret, Hartman, Looney, Bertinetti-Lapatki, Rohr, Hickling, Kraft and Marban-Doran

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of the experimental procedure to assess the contribution of dendritic cells to immunogenicity. The common starting point of the assays is the PBMC isolation according to standard protocols. CD14+ cells isolation and differentiation into immature moDCs are also shared between the procedures. Immature moDCs are challenged with the treatment as described in Material and Methods for the DC internalization and DC activation assays. Matured moDCs are used for MHC-II Associated Peptide Proteomics (MAPPs).