Table 1.
Cell stimulations with 24 h incubation in vitro (n per set-up).
| BALC | Medium | P/i a | Asp f 1 b | Asp f 7/8 b | f-t c |
|---|---|---|---|---|---|
| HE | 9 | 9 | 9 | 9 | 9 |
| MEA | 3 | 3 | 3 | 3 | 3 |
| SEA | 5 | 5 | 5 | 4 | 5 |
| PBMC | Medium | P/i a | Asp f 1 b | Asp f 7/8 b | f-t c |
| HE | 7 | 7 | 7 | 5 | 7 |
| MEA | 3 | 3 | 3 | 2 | 3 |
| SEA | 8 | 8 | 8 | 7 | 8 |
a
P/i PMA and ionomycin were added after 18 h, for the last 6 h of the total 24 h incubation in vitro;
b
Aspergillus antigens were recombinantly produced in E. coli and purified as described previously (23, 24);
c
f-t cells were kept on ice for 6 h after thawing and analysed as controls without stimulation.