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Antibodies used for flow cytometry.
| Equine target molecule | Antibody clone | Fluorochrome | Reference of equine-specific binding |
|---|---|---|---|
| CD4 | HB61A | DyLight488 a | (35) |
| CD8 | CVS8 | DyLight405 a | (35) |
| IL-17 | 76 | AlexaFluor647 a | (29) |
| IL-4 | 13G7 | AlexaFluor555 a | (36) |
| IFN-γ | 38-1 | Biotin
a
, followed by PE-Cy7 streptavidin (BioLegend) |
(37) |
conjugated in-house using Sulfo-NHS esters (Thermo Fisher Scientific).
