FIGURE 4.

IL-6 basal release and gene expression in ALI-PBEC. PBECs differentiated using ALI-culture were pre-treated with NAC or MIX for 2 h and then stimulated with CSE 20%. After 24 h, basal medium was collected and total RNA was extracted to evaluate IL-6 protein release and gene expression. (A) IL-6 protein release measured by ELISA. Results are reported as pg/mL (N = 9). *p < 0.05 ANOVA (Fisher’s) test. (B) IL-6 gene expression measured by RT-PCR. Results are reported as mean fold change compared to control ±SD (N = 8). *p < 0.05 ANOVA (Fisher’s) test.