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. 2024 Aug 20;14:1455259. doi: 10.3389/fcimb.2024.1455259

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Copyright © 2024 Jang, Kwon, Jang, Lee, Chang, Jeon, Jeong, Song, Min, Park, Lee, Han, Yang, Lee and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant FopA protein expression and mouse immunization. (A) Schematic illustration of the expressed domains of FopA protein with polyhistidine tags and amino acid sequence of FopA. (B) The production of recombinant FopA protein was analyzed using SDS-PAGE. The harvested cell culture fluid (Lane 1), flow-through fraction (Lane 2), washed fraction (Lane 3), and elution fraction (Lane 4). (C) Schematic representation of the mouse immunization protocol. The mice were immunized four to five times at 2-week intervals, and serum was collected after the second boost step for serum antibody titration. The spleen was collected 3 days after the final boost. Created with BioRender.com.