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. 2024 Aug 20;14:1455259. doi: 10.3389/fcimb.2024.1455259

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Copyright © 2024 Jang, Kwon, Jang, Lee, Chang, Jeon, Jeong, Song, Min, Park, Lee, Han, Yang, Lee and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibody screening with Immune library and Hybridoma technology. (A) Schematic representation of the Hybridoma antibody screening method and phage display screening method with immune antibody library. (B, C) Screening of antibodies against the FopA recombinant protein. Out of the 954 antibody clones produced from hybridoma screening, 6H6 and 6D10 (O.D. 450nm > 1.0) were selected. 16 positive clones (O.D. 450nm > 1.0) were selected from the total of 672 scFv clones produced by the immune library. (D, E) Sequence identification of the variable heavy (VH) and light (VL) chains of anti-FopA antibodies. The VH and VL regions of each antibody were sequenced to identify the complementarity-determining regions (CDRs). Created with BioRender.com.