Figure 5.

Detection of FopA using the sandwich ELISA in various matrices. The evaluation of a pair of antibodies for detecting recombinant FopA using sandwich ELISA in various matrices, including 3% human serum, 3% bovine serum albumin (BSA), mouse urine, and soil water. (A) The capture monoclonal antibody (50 nM) coated on a 96-well ELISA plate and biotinylated detector monoclonal antibody (20 nM) were employed to assess the FopA concentration serially diluted from 20 ng/mL. The detection sensitivity of FopA was evaluated by serial dilution of the sample, resulting in a determined limit of detection (LoD) of 0.066 ng/mL for 3% human serum (B), 0.074 ng/mL for 3% BSA (C), 0.071 ng/mL for mouse urine (D), and 0.067 ng/mL for soil water (E), and a red dot indicating the background signal. Error bars represent standard deviations from a triplicate.