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. 2024 Jul 25;31(9):1170–1183. doi: 10.1038/s41418-024-01346-x

Fig. 8. Deficient Mtgr1−/− ISCs can be rescued by high Wnt stimulation.

Fig. 8

A ISC cells identified by scRNA-seq were queried for expression of ISC associated genes. B ISC-specific transcriptomic signatures were assessed by GSEA using the WikiPathways_2019_Mouse collection. Subset of statistically significant gene sets/pathways shown here (all significant pathways shown in Supplementary Fig. 7) for WT and (C) Mtgr1−/− ISCs. D WT and Mtgr1−/− enteroids were plated with the indicated amounts of CHIR. Enteroid numbers were assessed daily and normalized to day 1 results. n = 7 WT and 10 Mtgr1−/− control samples, 4 samples for all other genotypes/conditions. ***P < 0.001, ****P < 0.0001, Mann–Whitney Wilcoxon test (A), significance indicated by FDR q value (B, C), or two-way ANOVA (D).