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. 2024 Sep 3;10:392. doi: 10.1038/s41420-024-02158-1

Fig. 5. The targeting of SLC1A5 by miR-125b-5p in HNSCC cells.

Fig. 5

A Illustration of miR-125b-5p targeting site in SLC1A5 3’UTR. The complementarity between miR-125b-5p and SLC1A5 in wild-type reporter (WT) is abolished in the mutated reporter (MUT). B reporter assay by co-transfecting VA, SLC1A5 WT, and SLC1A5 MUT vector with or without miR-125b-5p mimic. C, D Lt panels, upregulation or downregulation of miR-125b-5p by transfecting mimic or inhibitor reagents. Rt panels, western blot analysis to show the decrease or increase of SLC1A5 protein expression following the treatment with mimic or inhibitor, respectively. Numbers designate the normalized expression value. Mann-Whitney test. * and **, P < 0.05 and P < 0.01, respectively.