Fig. 6.

9B8 Inhibits IL-1β-induced Enhancement of Chondrocyte Inflammation via the HIF1 Signal Pathway.

A. Primary human chondrocytes were treated with varying concentrations of 9B8 (0, 50, 100, 200 μg/ml) and IL-1β (10 ng/ml). After 24 h of incubation, unsupervised hierarchical clustering analysis was performed to illustrate the differential mRNA expression using heat maps, where red indicates up-regulation and green indicates down-regulation. B. Alterations in the expression of signaling pathway between groups. C. Relative mRNA expression of PFKP, SLC2A1, and ENO2 in chondrocytes stimulated with IL-1β (10 ng/ml) and various concentrations of 9B8 (0, 50, 100, 200 μg/ml) at 24 h, measured by qRT-PCR. D. Protein levels of PFKP and ENO2 in chondrocytes treated with IL-1β (10 ng/ml) and different concentrations of 9B8 (0, 50, 100, 200 μg/ml) were analyzed using Western blot. All experiments were conducted at least three times. Data are presented as mean ± standard deviation. Significance levels are denoted as × p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared with IL-1β treated groups.