Fig. 2. Engineered cellular biosynthesis of UDP-GalNAzMe and UDP-GalNPrAz in K-562 cells. (A) Experimental layout and synthetic compounds used. (B) Biosynthesis of UDP-GalNAzMe in stably transfected cells as measured by HPAEC. Cells were fed with 50 μM synthetic compounds or DMSO. Data are from one representative out of at least two replicates. Asterisk denotes a peak likely due to an artifact of chromatography conditions. (C) Biosynthesis of UDP-GalNPrAz in stably transfected cells as measured by HPAEC. Cells were fed with 50 μM compound 2 or DMSO. Data are from one representative out of at least two independent replicates.
