Fig. 4.
JAK-STAT and BMP signaling pathway components have increased expression in Set1 knockdown testis. (A) Volcano plot of differentially expressed genes in Set1 KD testes versus Ctrl KD testes at 3 days post-eclosion (≥log21.3=2.46-fold change, P<0.05). Non-DE, not differentially expressed. (B) GO analysis of the 1216 upregulated genes identified from A. GO analysis of the 764 downregulated genes did not show any significant category. (C) Several JAK-STAT and BMP signaling pathway components that are upregulated in Set1 KD testes compared with Ctrl KD testes 3 days post-eclosion. (D) Expression levels of Mad and Stat92E in Set1 KD testes versus Ctrl KD testes. Error bars represent s.e.m., n=3 biological replicates, *P<0.05 using unpaired t-test. TPM, transcripts per million reads.