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. 2024 Apr 29;5(8):100930. doi: 10.1016/j.xplc.2024.100930

Figure 4.

Figure 4

PHR2 enhances the growth of Pseudomonas sp. (P6) through biosynthesis of succinic acid and methylmalonic acid in rice roots.

(A) Volcano plot of differentially abundant metabolites between WT and OsPHR2 OE rice root exudates. VIP, variable importance in projection.

(B) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enriched in the differentially abundant metabolites between WT and OsPHR2 OE rice root exudates.

(C) Heatmap of different organic acids between WT and OsPHR2 OE rice root exudates. VIP, variable importance in projection.

(D and E) Relative abundance of succinic acid and methylmalonic acid in WT and OsPHR2 OE rice root exudates. Data are means ± SD (n = 3). Asterisks indicate significant differences determined by two-sided Student’s t-test (∗p < 0.05 and ∗∗p < 0.01).

(F) Growth of strain P6 with addition of succinic acid or methylmalonic acid. Data are means ± SD (n = 5). Asterisks indicate significant differences determined by two-sided Student’s t-test (∗p < 0.05).