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. 2024 Aug 20;6(5):fcae282. doi: 10.1093/braincomms/fcae282

Figure 2.

Figure 2

GR activates the NLRP3 inflammasome in hippocampal slice cultures. Mouse hippocampal slice cultures (from male and female mice) were LPS-primed and treated with GR ± MCC950 for 24 h (n = 3). AP treatment was also performed (n = 2). IL-1β concentrations in media collected from hippocampal slices 4 h (A) and 24 h (B) post-treatment. Cell death measured by LDH assay of media collected from hippocampal slices 4 h (C) and 24 h (D) post-treatment. (E) Immunofluorescence imaging of ASC in hippocampal slices. ASC specks are visible as intense puncta in the cytosol of cells in GR-treated hippocampal slices (top right). (F) Quantification of total ASC specks per field of vision. All values are mean ± SEM. Data were analysed by one-way ANOVA with post hoc Dunnett’s or Tukey’s tests for multiple comparisons. The scale bars represent 50 µm. Individual mice were considered the experimental unit.