Figure 6.

Antigen presentation by BM-derived APCs is critical for tumor treatment. (A) Generation of BM chimeras. We generated chimeras by transferring 5 × 10⁶ BM cells from β₂m^–/– and WT mice into lethally irradiated WT or β₂m^–/– mice. (B–D) Results from BM chimeras indicate that BM-derived APCs expressing MHC class I are required for tumor treatment. Chimeras bearing 10-day-old established s.c. B16 tumors were sublethally irradiated and left untreated as controls or received adoptive transfer of 1 × 10⁶ pmel-1 cells cultured for 1 week in conjunction with rFPhgp100 vaccination and exogenous rhIL-2 (36 μg per dose). Results for tumor area are the mean of measurements from 5 mice per group (± SEM). Data shown are representative of 2 independent experiments.