Table 2.
Primers sequences used for qRT-PCR analysis
| Gene symbol | Amplicon size (bp) | Gene description | Accession number/REF. | Primer Sequence | |
|---|---|---|---|---|---|
| 1 | House keeping gene: | ||||
| β-actin (ACTB) | 177 bp | Beta-actin | L08165.1 |
F:- 5′-CCCACACCCCTGTGATGAAA-3′ R:- 5′-TAGAACTTTGGGGGCGTTCG-3′ |
|
| 2 | Heat stress responsive gene: | ||||
| HSP-70 | 145 bp | Heat shock protein − 70 | Calik et al., 2022 [1] |
F: 5′-CGTCAGTGCTGTGGACAAGAGTA-3′ R: 5′-CCTATCTCTGTTGGCTTCATCCT-3′ |
|
| 3 | Gut health biomarker: | ||||
| I-FABP-2 | 77 bp | Intestinal fatty acid binding protein 2 | Chen et al., 2015 [140] |
F:5-′AAAGATAATGGAAAAGTACTCACAGCAT-3′ R:5′-CCTTCGTACACGTAGGTCTGTATGA-3′ |
|
| 4 | Immunity related genes: | ||||
| IL-10 | 174 bp | Interleukin-10 | NM_001004414.4 |
F: 5′- TGTACCATTTGTGGCAGTGC − 3′ R: 5′- TCGTCTGGTGTTTGCAGTTG − 3′ |
|
| TLR-4 | 158 bp | Toll-like receptor 4 | NM_001030693.1 |
F: 5′-ATGTCCTCTTGCCATCCCAA-3′ R: 5′-TCTCCCCTTTCTGCAGAGTG-3′ |
|
| 5 | Nutrient sensing pathway: | ||||
| mTOR | 213 bp | Mechanistic Target of Rapamycin | XM_417614.6 |
F: 5′-CGCAGTGAAGAAACAAGGGC-3′ R: 5′-GGTGGCGTTACCTCCTTCAA-3′ |
|