. 2024 Sep 3;20:388. doi: 10.1186/s12917-024-04233-2

Table 3.

Effect of phytogenic feed additives (PHY) on hematological parameters of thermoneutral, and heat-stressed broiler chickens

Parameters	TN	TN + PHY	HS	HS + PHY
Hematocrit (%)	37.8 ± 0.37 ^a	38.2 ± 0.58 ^a	39.4 ± 0.51 ^a	38.4 ± 0.51 ^a
Hb conc (g/dl)	7.82 ± 0.06 ^a	8.16 ± 0.29 ^a	7.96 ± 0.06 ^a	7.96 ± 0.08 ^a
RBCs count (* 10⁶ cell/mm³)	2.77 ± 0.04 ^b	2.85 ± 0.04 ^b	3.58 ± 0.1 ^a	2.88 ± 0.04 ^b
MCV (fl.)	136.74 ± 0.93 ^a	134.04 ± 0.47 ^ab	110.25 ± 1.71 ^c	133.35 ± 0.52 ^ab
MCH (pg)	28.31 ± 0.85^a	28.59 ± 0.64 ^a	22.3 ± 0.49 ^b	27.65 ± 0.18 ^a
MCHC (%)	20.7 ± 0.09 ^ab	21.33 ± 0.49 ^a	20.22 ± 0.15 ^b	20.73 ± 0.12 ^ab
WBCs count (* 10³ cell/mm³)	11.08 ± 0.31 ^b	11.30 ± 0.24 ^b	14.96 ± 0.39 ^a	11.89 ± 0.32 ^b
Heterophile (H) %	29.81 ± 0.71 ^b	29.47 ± 0.51 ^b	41.23 ± 1.5 ^a	31.10 ± 0.33 ^b
Lymphocyte (L) %	65.19 ± 0.64 ^a	65.53 ± 0.49 ^a	53.77 ± 0.9 ^b	63.90 ± 0.32 ^a
H/L	0.46 ± 0.02 ^b	0.45 ± 0.01 ^b	0.77 ± 0.05^a	0.48 ± 0.008 ^b

^{a, b, c} Means within a row with different superscripts significantly differ (Tukey’s test; P < 0.05)

TN (control): Thermoneutral treatment, fed basal diet; TN + PHY: Thermoneutral treatment fed basal diet + 1 kg/ton feed PHY; HS: Heat stress treatment, fed basal diet; HS + PHY: Heat stress treatment, fed basal diet + 1 kg/ton feed PHY.

Hb conc: Hemoglobin concentration; RBCs count: Red blood cells count; MCV: Mean corpuscular volume; MCH: Mean corpuscular hemoglobin; MCHC; Mean corpuscular hemoglobin concentration; WBCs count: White blood cells count; (H/L) ratio: heterophile/ lymphocyte ratio

Data are means ± SEM (standard error of the mean)

Number of sampled birds (n) = 3 birds/replicate (12 birds/ treatment)