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Schematic representation of the viability PCR (v-PCR). Propidium monoazide (PMA) binds to free RNA. RNA bound to PMA is not amplifiable in the PCR, while RNA of intact virus is unaffected by PMA and can be amplified with PCR. The delta cycle threshold (ΔCt) value is calculated by subtracting the Ct value of the PMAxx-treated sample from the Ct value of the non-PMAxx-treated sample.
