Fig 4.

Effect of C-POLAR on the viability of bacterial pathogens. Gram-positive (Staphylococcus aureus and Enterococcus faecalis) or Gram-negative (Pseudomonas aeruginosa and Escherichia coli) were directly inoculated into media (control), control fabric, or 6% C-POLAR-coated textile, incubated for 10 min at room temperature and then 450 µL of complete RPMI media (RPMI with 10% FBS, l-glutamine, without antibiotics) was added to the fabric patches in the same microfuge tube. Media only (no textile or bacteria) served as a background control. The inoculated textile was incubated by shaking at 37°C for 3 h, at which point the turbidity was measured from the suspension at OD₆₀₀nm. Asterisks indicate statistically significant differences between the control (untreated) and C-POLAR-treated-textile determined by a two-tailed t test, **P < 0.02, ***P < 0.004, and ****P < 0.0001.