. 2024 Jul 30;12(9):e01164-24. doi: 10.1128/spectrum.01164-24

TABLE 2.

List of nucleotide and amino acid mutations in HN protein with >50% variant frequency^e^,f

Species	Culture (passage)	Name	Nucleotide	Reference	Allele	Frequency			Amino acid	Reference	Mutation^a	Amino acid variation (number)^b	n ^c
Species	Culture (passage)	Name	Nucleotide	Reference	Allele	P1	P3	P5	Amino acid	Reference	Mutation^a	Amino acid variation (number)^b	n ^c
Respirovirus laryngotracheitidis (human parainfluenza virus 1)	HBTEC-ALI	PIV1_Fukushima_O503_2019	8449	G	A	-	44.5	74.3	524	G	R	G (491), V (6), E (2)	499
	Vero/TMPRSS2	PIV1_Fukushima_H171_2018	7531	C	T	-	78.3	99.4	217	A	V	A (479)	479
		PIV1_Fukushima_H171_2018	8421	A	G	-	-	94.6	514	K	E	K (472), E (23), R (5)	500
		PIV1_Fukushima_O425_2018	8236	A	G	-	26.4	67.3	453	K	E	K (289), R (244), E (8), Q (1)	542
		PIV1_Fukushima_O503_2019	7646	A	G	-	99.6	97.3	256	K	R	K (479), R (4), N (1), I (1)	485
Orthorubulavirus laryngotracheitidis (human parainfluenza virus 2)	Vero/TMPRSS2	PIV2_Fukushima_O836_2019	7274	G	A	-	-	71.2	155	S	N	S (187)	187
	Vero	PIV2_Fukushima_O33_2018	7535	G	A	-	-	72.1	239	G	E	G (236), R (1)	237
		PIV2_Fukushima_O836_2019	7337	A	G	-	-	82.3	176	K	R	K (185), R (1), N (1)	187
		PIV2_Fukushima_O861_2019	7404	A	G	-	33.6	94.1	197	N	S	N (206), S (1), D (1)	208
Respirovirus pneumoniae (human parainfluenza virus 3)	Vero/TMPRSS2	PIV3_Fukushima_O330_2018	8471	A	G	-	99.4	99.8	556	N	D*^d	dN (1418), D* (12), I (2), B (1), T (1)	1434
		PIV3_Fukushima_O644_2019	7493	A	G	-	89.5	71.1	230	T	A	T (1814), A (4)	1818
		PIV3_Fukushima_O726_2019	7379	A	G	-	97.0	98.5	192	R	G	R (1612), G (5), K (5), I (3)	1625
		PIV3_Fukushima_O726_2019	7451	G	A	84.4	98.7	99.2	216	D	N*^d	D (1741), E (3), N* (2), G (1), B (1)	1748
Orthorubulavirus hominis (human parainfluenza virus 4)	Vero/TMPRSS2	PIV4a_Fukushima_H725_2019	8049	A	T	NA	75.4	84.3	160	Y	F	Y (131)	131
		PIV4b_Fukushima_O896_2019	8555	C	T	NA	-	61.3	332	S	L	S (151)	151
		PIV4b_Fukushima_OR476_2022	9022	C	A	84.9	81.8	79.5	487	A	E	A (127)	127

^{^a}

We searched the registered HN protein sequences that had the indicated amino acid, and if it was also included in the direct sequence from a clinical specimen without growth in cultured cells, it is indicated by an asterisk.

^{^b}

Amino acid variations were examined in all registered HN protein sequences, and the number of registered sequences having the amino acid residues is shown.

^{^c}

Number of registered HN protein sequences assigned for comparison.

^{^d}

GenBank accession nos. QZB59302 (D) and BBL33137 (N).

^{^e}

Bold entries show amino acid mutations with no variation that were unique to the five passages.

^{^f}

NA, not analyzed due to insufficient coverage; -, not detected.