TABLE 3.

List of nucleotide and amino acid mutations other than HN protein with >50% variant frequency

Gene	Culture (passage)	Name	Nucleotide	Reference	Allele	Frequency			Amino acid	Reference	Mutation
						P1	P3	P5
P	HBTEC-ALI	PIV3_Fukushima_O726_2019 (C protein)	2090	G	C	-a	17.2	52.9	103 (99)	E (R)	Q (S)
	Vero/TMPRSS2	PIV1_Fukushima_O425_2018	1977	A	C	-	30.1	66.6	53	I	L
		PIV3_Fukushima_O644_2019	2420	G	A	-	86.7	70.8	210	D	N
M	Vero/TMPRSS2	PIV1_Fukushima_O425_2018	4273	G	A	-	70.4	68.6	210	V	M
		PIV2_Fukushima_O861_2019	3832	G	A	-	60.7	52.9	119	S	N
F	Vero/TMPRSS2	PIV3_Fukushima_O644_2019	5242	A	G	-	11.6	68.4	57	I	M
L	Vero/TMPRSS2	PIV1_Fukushima_H171_2018	14571	A	G	-	80.2	99.4	1941	R	G
		PIV1_Fukushima_O425_2018	13979	A	G	-	22.9	64.5	1744	E	G
		PIV1_Fukushima_O503_2019	14055	A	C	-	58.0	82.8	1769	Q	H
		PIV1_Fukushima_O503_2019	14454	G	A	-	99.8	99.6	1902	M	I
		PIV2_Fukushima_O33_2018	14477	C	T	-	40.3	63.2	1894	S	L
		PIV2_Fukushima_O836_2019	12281	A	T	-	29.5	88.0	1165	E	V
	Vero	PIV2_Fukushima_O33_2018	13475	C	T	-	46.5	74.3	1560	P	L
		PIV2_Fukushima_O836_2019	11898	G	T	-	-	82.3	1037	K	N
		PIV2_Fukushima_O836_2019	15167	G	A	-	-	83.7	2127	G	D

^a -, not detected.