Table 2.

C₅₀ values (μM) determined from quantitative analysis of the DNase I footprinting experiments with TFO-1

	C50 (μM)
	pH 5.0	pH 6.0	pH 7.0
Perfect match	<0.01	0.011 ± 0.002	1.1 ± 0.1
S.AT	<0.01	0.020 ± 0.005	4.5 ± 0.7
BAU.TA	0.013 ± 0.002	0.04 ± 0.02	n.d.
MeP.TA	0.019 ± 0.005	n.d.	n.d.
APP.TA	a	a	n.d.

The target sites differed by a single base pair within the 19mer target site, generating a triplex mismatch opposite one of the novel nucleotides. The identity of the mismatch (S.AT, BAU.TA, ^MeP.TA or ^APP.TA) is indicated. The experiments were performed at pH 5.0, 6.0 and 7.0 in an appropriate buffer containing 50 mM NaCl. n.d. indicates that no footprint was detected.

^aFor this target, TFO-1 only produced a partial footprint that did not cover the entire site.