Figure 2. Deep snRNA-sequencing resolves twelve small subpopulations of candidate thermo- and hygrosensors.

(A) Location of Ir93a+ thermo- and hygrosensory neurons on segments 1, 2, and 13 of female Ae. aegypti antennae^19,21. (B) Annotated UMAP showing reclustering of 589 nuclei from two Ir93a+ clusters in the original analysis (Fig. 1D). Note that 2 of the 12 new clusters do not expres Ir93a. (C) Dotplot showing expression of 1571 genes differentially expressed across clusters (log2FC>0.3). (D) Histogram showing number of nuclei in each cluster (grey bars) overlaid by the expected densities for cell types comprising 1, 2, or 3 neurons per antenna given 23X coverage (red lines).