Fig. 2.

Neutralization of gC-null mutant HSV-1 infection of Vero cells by antibodies to gD. HSV-1 gCR (black) or HSV-1 ΔgC (red) (100 PFU) was treated with monoclonal antibodies MC23 (A), DL11 (B), MC14 (C), MC5 (D), 1D3 (E), or H170 (F) for 1 h at 37°C. Infectivity was determined by plaque formation on Vero cells. Each experiment was performed with triplicate samples. Values are the means and standard errors of results from three independent experiments. Statistical significance was determined via Student’s t-test where *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. (G) Antibody concentration at which > 50% of virus was neutralized. Fold difference was calculated by dividing the concentration of MAb required to neutralize HSV-1 gCR by the concentration of MAb required to neutralize ΔgC.