Figure 3. Enhancer features predictive of functional activity.

(A) Comparison of molecular features between On-Target and other enhancer categories. ** P < 0.001, *** P < 0.0001 Wilcoxon rank-sum test two-sided, unpaired.

(B) Correlation of H3K27ac and ATAC-seq specificity for astrocyte enhancers.

(C) Examples of astrocyte enhancers with in vivo activity that is better predicted by H3K27ac than ATAC-seq signal.

(D) Summary of informative features from a Random Forest model predicting enhancer activity. ANOVA with Tukey post hoc tests, Bonferroni-corrected P-values.