Figure 1. Overview of the enhancer prioritization challenge.

(A) Single nucleus multi-omic data from primary motor cortex of human, macaque, marmoset, and mouse. mya, million years ago.

(B) Schematic of the computational challenge to prioritize candidate cell type-specific enhancers.

(C) Overview of AAV construction, cell type ATAC-seq specificity, and screening of in vivo activity in the mouse brain for three candidate L5 ET enhancers.

(D) Teams predicted and ranked 10,000 candidate enhancers for each of 19 cortical cell types and were scored based on prioritization of strong, On-Target enhancers.

(E) Combinations of data and methods for top team submissions.

(F) Normalized benchmark metrics (Methods) based on epifluorescence and SSv4 from in vivo screening.