Fig. 1. TDP43 mislocalizes to autophagosomes, lysosomes, and stress granules upon treatment with GU-rich oligonucleotides.
(A) Schematic of iPSC differentiation into forebrain-like neurons and subsequent (GU)6 oligonucleotide treatment. (B-E) Representative confocal microscopy images with xz and yz orthogonal views of (GU)6-treated DIV10 iNeurons fixed and immunostained for MAP2 (gray), TDP43 (magenta), and (B) LC3B, (C) p62, (D) LAMP2, or (E) G3BP1 (cyan). Scale bar, 5μm. Insets provide ~4.8x magnification; scale bar, 1μm. White arrowheads in xz and yz orthogonal views indicate TDP43 puncta present in inset.