Figure 3. Dlk1 is re-expressed in a murine model of ACC and exhibits intratumoral heterogeneity.

A-F) Immunohistochemical detection of Dlk1 expression in ACC from BPCre male (A) and female (B) mice with low Weiss Score, in metastatic ACC (C, primary male; E, primary female) and in lungs metastasis (Mets, D, males; F, females). Note the higher expression of Dlk1 in metastatic ACC and lungs metastasis. Dlk1 expression is mostly clonal with different foci that express varying levels of Dlk1 or be negative for Dlk1 expression. G) Dlk1 expression increases in a stepwise manner from non-metastatic primary ACC to metastatic primary ACC and metastatic lesions. Horizontal lines represent group means. H) Dlk1 expression positively correlates with age, which in turn increases with disease malignancy. Each dot represents an individual tumor. *p<0.05, **p<0.01, ***p<0.001.