Figure 3.
Inhibition of L. cremoris LAC277 by LysL from recombinant L. lactis N8 and wild-type L. lactis LAC460 concentrated CFSs (A) and cell lysates (B). 10 µl of the lysates/20-fold concentrated CFSs were spotted onto L. cremoris LAC277 indicator lawn on M17GE and incubated overnight at 30°C. LysL, L. lactis N8 transformed with the lysL expression plasmid; SPUsp45–LysL, L. lactis N8 transformed with the SSusp45–lysL expression plasmid; LAC460, L. lactis LAC460; and control, L. lactis N8 transformed with pVS2. A clearer and larger halo was created by the CFS of LysL producing L. lactis N8 than that of SPUsp45–LysL producer. The cell lysate containing LysL caused a clear inhibition zone whereas the SPUsp45–LysL lysate created a very diffuse halo. In case of LAC460, only CFS gave inhibition halo. The figure was digitally processed to enhance their quality.