Figure 4.
Autolysis of the LysL-producing recombinant L. cremoris NZ9000 cultures before and 80 min after induction by nisin. LysL, L. cremoris NZ9000 producing native LysL; SPUsp45–LysL, L. cremoris NZ9000 producing SPUsp45–LysL; and control, L. cremoris NZ9000 carrying plasmid vector pVS2. After 5 h (OD600 about 0.5) incubation at 30°C, the cultures were induced by nisin (0.5 IU/ml) followed by 80 min incubation. Native LysL-producing culture turned completely transparent, whereas the SPUsp45–LysL-producing culture remained partly clear. The control strain culture without any LysL production became turbid. The figure was digitally processed to enhance their quality.