Figure 5.

(A) Coomassie blue-stained SDS-PAGE visualizing proteins produced in the cell-free translational system using an RTS 100 E. coli HY Kit. Lane 1, reaction solution using lysL as a template; Lane 2, reaction solution without template as a control; and M, PageRuler Protein Ladder (Thermo Scientific). The figure was digitally processed to enhance their quality. (B) Western blot analysis of the cell lysate of the culture of E. coli BL21(DE3) carrying pET-22b(+)-lysL. The His-Tag antibody used recognized LysL containing a 6 × His-Tag in the N-terminus (lane 1), M, PageRuler Broad Range Protein Ladder (Thermo Scientific).