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. 2024 Aug 22;18(1):wrae161. doi: 10.1093/ismejo/wrae161

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© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of the infection dynamics at two different temperatures (3°C and 7°C). (A) MpoV particle abundances during single infection at 3°C (dotted line, open symbol) vs 7°C (solid line, closed symbol). (B) MpoV-46T viral genome production at 3°C vs 7°C. (C) MpoV-45T viral genome production at 3°C vs 7°C. Viral genome production is min-max normalized with the respective single virus infection treatment as reference. (D) MpoV particle abundances at 3°C vs 7°C during dual infection without delay (D0) and with 24 h delayed (D24) addition of MpoV-45T. (E, F) viral genome burst size (defined as the number of genome copies produced per lysed host cell; and expressed as percentage of single infection) of MpoV-46T (E) and MpoV-45T (F) at 3°C and 7°C during dual infection without delay (D0) and with 24 h delayed (D24) addition of MpoV-45T. Results are shown as mean ± SD (N = 3 except for 3°C single and non-delayed dual treatment where N = 6).