TABLE 2.
Analytical performance summary
| O-type | Strain | Target | PCR efficiency (%) | Linearity | Precision (%CV) | LoD | |
|---|---|---|---|---|---|---|---|
| Intra-assay | Inter-assay | (genomes/PCR) | |||||
| O1 | MGH KPN003 | O1/2 | 88.1 | 0.992 | 0.7–2.9 | 1.7–3.6 | 5 |
| O1wbbY | 92.9 | 0.992 | 0.4–2.1 | 1.2–2.2 | |||
| O2a | MGH KPN006 | O1/2 | 89.6 | 0.997 | 1.0–3.1 | 1.1–2.7 | 5 |
| O2afg | MGH KPN030 | O1/2 | 89.5 | 0.997 | 1.2–3.0 | 2.0–7.4 | 5 |
| O2afg | 99.7 | 0.998 | 0.9–2.4 | 1.7–2.8 | |||
| O3/O3a | MGH KPN047 | O3 | 89.7 | 0.996 | 1.5–2.4 | 1.7–3.9 | 5 |
| O3b | MGH KPN001 | O3 | 86.6 | 0.982 | 2.1–3.8 | 2.3–3.0 | 5 |
| O3b | 99.8 | 0.994 | 1.1–3.6 | 2.0–3.0 | |||
| O5 | MGH KPN049 | O5 | 97.3 | 0.996 | 1.2–2.5 | 1.5–2.5 | 10 |