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. 2024 Jul 2;5(8):1154–1166. doi: 10.34067/KID.0000000000000491

Figure 6.

Figure 6

Neutrophils in kidneys of patients with LN and controls. Kidneys from controls and patients with LN were co-immunostained for MPO (red staining) and CD66b (green staining). Nuclei were stained with DAPI. (A) Glomerulus from a control (panels 1–4) shows an intact PMN (white arrow) expressing MPO and CD66b (colocalization shown in yellow). Glomeruli from patients with LN also contain intact PMNs (panels 5–8; white arrows) with MPO and CD66b colocalized within cells, as well as areas with dispersed/diffuse MPO staining, suggestive of extracellular localization (panels 9–12; white arrowheads). (B) Neutrophils positive for MPO and CD66b staining are present in the interstitial regions (white asterisk, top row) and inside tubule lumen (white pound sign, bottom row) in kidneys of patients with LN. n=4/group. Single plane confocal images are shown. Scale bars: 30 µm. DAPI, 4’,6-diamidino-2-phenylindole; PMN, polymorphonuclear neutrophil.