Figure 8.

Localization of neutrophils and pattern of MPO staining within glomeruli of patients with LN and controls. To correlate neutrophil localization in glomeruli and MPO staining with glomerular histology, kidney sections from patients with LN and controls were immunohistochemically stained for MPO, followed by PAS staining of the section to define glomerular structure and pathology. (A) Glomeruli from controls show intact neutrophils (brown cells) contained within glomerular capillary loops (green arrows). Glomeruli from patients with LN contain intact neutrophils in capillary loops (green arrows) and the mesangial matrix or transmigrated from capillary lumen (yellow arrow). Glomeruli from patients with LN show diffuse/extracellular MPO staining around capillary loops or the mesangial matrix (green arrowheads). (B) To more clearly show the pattern of MPO staining in glomeruli, brown MPO staining was detected with Image Pro software in the same images as shown in (A), and the detected MPO staining area is highlighted in Cyan. Images are representative of n=4/group. Original magnification, 100×. Scale bar: 50 µm. PAS, periodic acid–Schiff.