Table 1.
List of apoplastic protein precursors discussed in the manuscript, with their domain lengths, their cognate proteases, and the subcellular compartments where their processing occurs.
| Symbol | Full length (aa) | Pro region (aa) | Mature domain (aa) | Protease involved in cleavage | Processing compartment | References |
|---|---|---|---|---|---|---|
| pro-Systemin (LOC543989) | 200 | 178; 4 | 18 | SlPhyt1; SlPhyt 2; LapA | Pearce et al., 1991; Gu and Walling 2000; Beloshistov et al., 2018 | |
| pre-pro-HypSys (LOC543883) | 146 | 24; 4; 25; 16 | 18; 15; 15 | apoplast | Pearce and Ryan 2003; Chen et al., 2008 | |
| pro-PEP1 (AT5G64900) | 92 | 69 | 23 | MC4 to MC9 | intracellular | Huffaker et al., 2006; Shen et al., 2019 |
| pre-pro-PIP1 (AT4G28460) | 72 | 29 | 13 | apoplast | Miyashita et al., 2011; Hou et al., 2014 | |
| pre-pro-PIP2 (AT4G37290) | 84 | 45 | 13 | apoplast | Hou et al., 2014; Vie et al., 2015; Hussain et al., 2021 | |
| pre-pro-SCOOP12 (AT5G44585) | 78 | 19 | 35 | SBT3.5 | apoplast | Hou et al., 2021b; Yang et al., 2023 |
| pre-pro-RALF23 (AT3G16570) | 138 | 60 | 50 | SBT6.1 | Srivastava et al., 2009; Xiao et al., 2019 | |
| pre-pro-PSK1 (AT1G13590) | 87 | 52; 6 | 5 | SBT3.8 | Matsubayashi et al., 2006; Amano et al., 2007; Stührwohldt et al., 2021 | |
| pre-pro-PSK4 (AT3G49780) | 79 | 49; 4 | 5 | SBT1.1 | Matsubayashi et al., 2006; Amano et al., 2007; Srivastava et al., 2008 | |
| pre-pro-PSY1 (AT5G58650) | 75 | 25; 10 | 18 | Amano et al., 2007; Matsubayashi 2011 | ||
| pre-pro-RGF9/GLV2 (AT5G64770) | 78 | 39 | 13 | Matsuzaki et al., 2010; Ghorbani et al., 2016; Kaufmann and Sauter 2019 | ||
| pre-pro-RGF7/GLV4 (AT3G02240) | 102 | 62 | 13 | Matsuzaki et al., 2010 | ||
| pre-pro-IDA (AT1G68765) | 77 | 23; 8 | 14 | SBT4.12; SBT 4.13; SBT 5.2 | Stenvik et al., 2008; Schardon et al., 2016; Stührwohldt et al., 2017 | |
| pro-ZIP1 (AC210027.3_FGP003) | 137 | 87; 33 | 17 | CP1; CP2 | apoplast | Ziemann et al., 2018 |
| PR1 (AT2G14610) (peptideCAPE9) |
161 | 124 | 11 | XCP1 | Chien et al., 2015; Chen et al., 2023b | |
| pre-pro-CLV3 (AT2G27250) | 96 | 48; 14 | 12 or 13 | intracellular | Rojo et al., 2002; Xu et al., 2013; De Marchis et al., 2018 | |
| pre-pro-PME17 (AT2G45220) | 511 | 148 | 307 | SBT3.5 | Senechal et al., 2014; Del Corpo et al., 2020 | |
| pre-pro-ARA-I (AY029259) | 777 | 130 | 615 | Lee et al., 2003 | ||
| pre-pro-SBT3.3 (AT1G32960) | 777 | 87 | 666 | self-activation | Ramírez et al., 2013; Coculo et al., 2023 | |
| pre-pro-RD21 (AT1G47128) | 462 | 115; 110 | 216 | Gu et al., 2012 | ||
| pre-pro-CDR1 (AT5G33340) | 437 | 48 | 364 | Xia et al., 2004; Simões et al., 2007 |
The indicated full aa lengths of the protein domains were predicted by UniProt (https://www.uniprot.org/). Sequences that do not belong to the mature domain are considered to be pro regions.