Figure 2.

(A) The design of multi-epitope construct with EAAAK, AAY and KK linkers. (B) The construct consists of 460 amino acids in which the linkers help join the epitopes with the adjuvant and with themselves. B-cell epitopes are represented in green, CTL epitopes in blue, HTL epitopes in red, and the adjuvant is in gray. (C) The secondary structure map, in which protein structures are coded in colors for the α-helix (pink), β-strands (yellow), and coil (gray). (D) The refinement of the model using the 3D-refine web server. (E) The docked model was obtained using ClusPro (https://cluspro.bu.edu). The lowest energy value obtained from the ClusPro calculation was −1072.5. Our designed construct is in pink and its affinity with the TLR2 receptor is evident. The three-dimensional images were created using RCSB PDB – 3D View (https://www.rcbs.org/3d-view).