Fig. 3.

Histologic evaluation of biopsies from aNRP perfused donor livers and technical aspects of the LiMAx test. A and B demonstrate the staining of liver biopsies with CYP1A2 immunohistochemistry. A consists of a low magnification image of 30 × , demonstrating that CYP1A2 is expressed in the hepatocytes in metabolic zone 2 (mid-lobular) and 3 (pericentral). B consists of a high magnification image of 100 × , demonstrating that the CYP1A2 enzyme is present in the cytoplasm of the hepatocytes. C demonstrates the percentage of the area that is positive on the CYP1A2 staining in the biopsies taken at the start of aNRP and at the end of aNRP in 5 donor livers. D shows an example of a typical LiMAx curve during aNRP. E demonstrates independence of the DOBmax from the blood flow during aNRP. F demonstrates the correlation between the measured total CO2 production and the estimated total CO2 production