Skip to main content
. 2024 Aug 1;56(8):1703–1716. doi: 10.1038/s12276-024-01279-y

Fig. 6. IL-3395–270 and IL-3399–270 are potent regulators of EC activation and MMP-2 activity.

Fig. 6

a–c Quiescent HRMVEC monolayers were treated with IL-3395–270, IL-3399–270, IL-33109–270, or IL-33112–270 for 4 h, and cell extracts were prepared and analyzed by Western blotting for the indicated proteins, followed by normalization to β-tubulin. d Quiescent HRMVEC monolayers were treated with IL-3395–270, IL-3399–270, IL-33109–270, or IL-33112–270 for 24 h, and the supernatant media and cell lysates were prepared. The cell lysates were analyzed by Western blotting for active ADAM10 and ADAM17 levels using specific antibodies, followed by normalization to β-tubulin. Gelatin zymography was performed to analyze MMP-2 activity in the supernatant media. The bar graphs show the quantitative analysis of 3 independent experiments, expressed as the mean ± SD. *p < 0.05 vs. control.