Fig. 3. Nuc1 regulates insertions of extranuclear DNA.

a–b Frequency and types of DNA inserted at DSBs in wild-type, nuc1Δ, and nuc1-H138A mutant cells in 8 days (a) or 3, 8 and 16 days (b) stationary phase cells. Pie charts demonstrate the distribution of different insertion donor DNA in wild-type and nuc1Δ cells at 8 days in stationary phase. n represents number of inserted DNA. P values were determined using two-tailed χ² test. n–number of NHEJ products tested is shown in Supplementary Data 1. c Survival and respiration proficiency of wild-type and nuc1Δ cells plated on rich media after 1–16 days in stationary phase, (mean ± SD; n = 3. n represents biological repeats). d Analysis of sequences inserted at DSB from the first 7 kb of mtDNA in wild-type and nuc1Δ cells (insertions from the complete mtDNA genome are shown in Supplementary Fig. 4). Single donor insertions were analyzed. e Sequences inserted into DSBs and the distribution of G4 forming sequences and GC content within the first 7 kb of mtDNA. Single donor insertions only were analyzed. f Analysis of number of donor DNA per insertion event in wild-type cells at 16 days in stationary phase. Number of insertions involving 1, 2, 3 or 4 DNA pieces are shown. g Distribution of different complex insertion events in wild type at 16 days. Mt-mitochondrial DNA, ty-retrotransposon DNA, ng- nuclear genome DNA. h Example of a complex NUMT. i Frequency and types of insertions in wild-type and nuc1Δ cells in young cells and aged mother cells. P values were determined using two-tailed χ² test. n-number of NHEJ products tested is shown in Supplementary Data 1. Source data are provided as a Source Data file.