Fig. 5. Nuc1 degrades retrotransposon cDNA replication intermediates.

a Analysis of Ty1 sequences inserted at DSBs in wild-type and nuc1Δ cells. Frequency of each Ty1 nucleotide insertion per 10⁶ NHEJ products or among all Ty1 nucleotides inserted. Schematic of Ty1 shown at top indicating long terminal repeats (LTR), primer binding site (PBS) and polypurine tracts (PPT1/2). b Frequency and types of DNA inserted at DSBs in wild-type, nuc1Δ, spt3Δ, and nuc1Δ spt3Δ mutant cells in 8 days stationary phase cells. Pie charts below demonstrate distribution of different insertion donor DNA. n represents number of inserted DNA. P values were determined using two-tailed χ² test. n–number of NHEJ products tested is shown in Supplementary Data 1. c Southern blot analysis of Ty1 cDNA in wild-type, nuc1Δ and control spt3Δ cells. Quantification of Ty1 cDNA smear is shown (mean ± SD; n = 4; n represents biological repeats. P values were determined using unpaired two-tailed Welch’s t-test). The position of the probe used is shown in (a). Source data are provided as a Source Data file.