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. 2024 Sep 3;10:79. doi: 10.1038/s41522-024-00558-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 9 — Mouse primary splenic NK cells were collected from alcohol-fed and treated mice and added to the apical side of a Transwell and migration in response to different chemokines was assessed following 5 h of incubation. A Confirmation of NK cell purification. (a1) Percentage of NK cells pre-isolation, and (a2) percentage of NK cells post-isolation. NK cell migration response profiles to (B) medial alone, (C) CCL2, (D) CXCL12, (E) CX3CL1, and (F) CCL9-11. Percent migration was calculated as the total number of viable NK cells in the bottom well divided by the total number of viable NK cells added to the Transwell insert. p values are indicated in the figure by one‐way ANOVA with Sidak’s multiple comparison. N = 6–7 wells of primary NK cells/group (12–14 mice/group, NK cells per 2 mice were pooled) derived from 2 independent experiments.