Figure 6.

Screening edible probiotic for high AMP production. (A) The rank of the edible probiotics for potential AMP intensity predicted by COMDEL. The possibility of edible probiotics to generated AMP was calculated and ranked by COMDEL according to the multi-omics data including genome, transcriptome and proteome. (B) The antibacterial activity verification of probiotic fermentation product against E. coli BL21 (DE3) and B. subtilis 168. (C) Schematic diagram of screening the L. plantarum mutants for high AMP production by FADS. A biosensor of the antibacterial activity coupled with the split GFP assembly is designed. (D) Antibacterial activity verification of the three screened L. plantarum mutants against seven microorganisms. (E) The genome map of the three screened L. plantarum mutants was drew by using the whole genome sequencing data. An asterisk (∗) indicates that the data are significantly different when comparing the OD₆₀₀ value of the condition with or without the addition of the fermentation supernatant.